One cannot help but to stand in awe of the rapid progress made in the medical field. New techniques and medicines are introduced at an astonishing rate. Of course, one of the main fields of research is diagnostics. No one will argue the fact that an early diagnosis ensures a better prognosis and lower treatment costs. ELISA kits, an enzyme linked immunosorbent assay, allow doctors to diagnose a variety of conditions quickly and efficiently.
The enzyme linked immunosorbent assay, or EIA as it is known for short is performed in a laboratory. The test is easy to run and many tests can be performed at the same time. The purpose of the test is to measure the concentration of both antigens and antibodies in the blood sample. The ability to measure this concentration is a very important part of the diagnostic process.
The EIA is deemed revolutionary because it is vital to be able to detect the presence of antibodies and antigens. When the body detects a harmful substance within the system it produces antibodies. The very fact that there are antibodies present means that there are harmful substances. These dangerous elements are called antigens. By identifying the type of antibody produced by the body, doctors can also determine the exact nature of the antigen that triggered the production of the antibodies.
Patients do not experience any form of discomfort during the test. A small sample of blood is removed and then sent to the laboratory. There, a technician will add the sample to a petri dish that is already prepared with antigens. When the antibodies in the blood sample bind with the specific antigen in the petri dish and identification can be made. Further enzymes are then added to see how the antibody and the antigen react to each other.
There are different variations of the EIA test but the more complex tests provide much more accurate results. Each type of test requires a different kit. The direct EIA test, for example is straightforward and results are available very quickly. In this case the antigens are coated with multiwall plates and the antibody can only react with the antigen once it has been conjugated with enzymes.
With the indirect EIA test polystyrene plate is used to cover the antigen. This allows the technician to process the test in two distinct stages before the antibody will be able to identify the antigen and react with it. During each stage it is possible to identify both primary and secondary antibodies. The benefit of this ability is that it saves money and time.
The sandwich EIA test requires the antibodies to be matched in pairs. The first one is covered with polystyrene and the role of the second antibody is to act as a detection agent. The main purpose of this test is to find out what the concentration of the analyte is. It is an extremely accurate test and can be used to diagnose latent diseases.
There can be little doubt that EIA test is an important tool that can help doctors to quickly and effectively diagnose a variety of diseases. The fact that the test even allow for the diagnosis of latent diseases means that many lives can be saved and treatment can be offered when there is still a good chance of success.
The enzyme linked immunosorbent assay, or EIA as it is known for short is performed in a laboratory. The test is easy to run and many tests can be performed at the same time. The purpose of the test is to measure the concentration of both antigens and antibodies in the blood sample. The ability to measure this concentration is a very important part of the diagnostic process.
The EIA is deemed revolutionary because it is vital to be able to detect the presence of antibodies and antigens. When the body detects a harmful substance within the system it produces antibodies. The very fact that there are antibodies present means that there are harmful substances. These dangerous elements are called antigens. By identifying the type of antibody produced by the body, doctors can also determine the exact nature of the antigen that triggered the production of the antibodies.
Patients do not experience any form of discomfort during the test. A small sample of blood is removed and then sent to the laboratory. There, a technician will add the sample to a petri dish that is already prepared with antigens. When the antibodies in the blood sample bind with the specific antigen in the petri dish and identification can be made. Further enzymes are then added to see how the antibody and the antigen react to each other.
There are different variations of the EIA test but the more complex tests provide much more accurate results. Each type of test requires a different kit. The direct EIA test, for example is straightforward and results are available very quickly. In this case the antigens are coated with multiwall plates and the antibody can only react with the antigen once it has been conjugated with enzymes.
With the indirect EIA test polystyrene plate is used to cover the antigen. This allows the technician to process the test in two distinct stages before the antibody will be able to identify the antigen and react with it. During each stage it is possible to identify both primary and secondary antibodies. The benefit of this ability is that it saves money and time.
The sandwich EIA test requires the antibodies to be matched in pairs. The first one is covered with polystyrene and the role of the second antibody is to act as a detection agent. The main purpose of this test is to find out what the concentration of the analyte is. It is an extremely accurate test and can be used to diagnose latent diseases.
There can be little doubt that EIA test is an important tool that can help doctors to quickly and effectively diagnose a variety of diseases. The fact that the test even allow for the diagnosis of latent diseases means that many lives can be saved and treatment can be offered when there is still a good chance of success.
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